Journal: Cells

Article Title: RAC1 , a Potential Diagnostic and Prognostic Marker for Diffuse Large B Cell Lymphoma

doi: 10.3390/cells11244039

Figure Lengend Snippet: Overall survival and the expression levels of Rho/Rac family genes in DLBCL. Kaplan–Meier curve of overall survival for individual member ( A ) RAC1, ( B ) RAC2, ( C ) RND1, ( D ) CDC42, ( E ) RHOQ, ( F ) RHOF; HR, hazard ratio.

Article Snippet: Sections from paraffin-embedded tissues were then immunostained with a rabbit polyclonal antibody against RAC1 (dilution: 1:300, Bioss, BJ, CHN).

Techniques: Expressing

Journal: Cells

Article Title: RAC1 , a Potential Diagnostic and Prognostic Marker for Diffuse Large B Cell Lymphoma

doi: 10.3390/cells11244039

Figure Lengend Snippet: Correlation between the expression of Rho/Rac family members’ and BTK. ( A ) Co-expression heatmap of Rho/Rac family genes and BTK; ( B ) Scatter diagram demonstrated the correlation between BTK and RAC1 , ( C ) CDC42, ( D ) RHOF and ( E ) RHOQ. * p <0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: Sections from paraffin-embedded tissues were then immunostained with a rabbit polyclonal antibody against RAC1 (dilution: 1:300, Bioss, BJ, CHN).

Techniques: Expressing

Journal: Cells

Article Title: RAC1 , a Potential Diagnostic and Prognostic Marker for Diffuse Large B Cell Lymphoma

doi: 10.3390/cells11244039

Figure Lengend Snippet: RAC1 expression levels in 18 different types of tumor tissues and paired adjacent normal tissues. * p < 0.05; ** p < 0.01; *** p < 0.001; ns: non-significance. BLCA, Bladder Urothelial Carcinoma; BRCA, Breast invasive carcinoma; CHOL, Cholangio carcinoma; COAD, Colon adenocarcinoma; ESCA, Esophageal carcinoma; HNSC, Head and Neck squamous cell carcinoma; KICH, Kidney Chromophobe; KIRC, Kidney renal clear cell carcinoma; KIRP, Kidney renal papillary cell carcinoma; LIHC, Liver hepatocellular carcinoma; LUAD, Lung adenocarcinoma; LUSC, Lung squamous cell carcinoma; PAAD, Pancreatic adenocarcinoma; PRAD, Prostate adenocarcinoma; READ, Rectum adenocarcinoma; STAD, Stomach adenocarcinoma; THCA, Thyroid carcinoma; UCEC, Uterine Corpus Endometrial Carcinoma.

Article Snippet: Sections from paraffin-embedded tissues were then immunostained with a rabbit polyclonal antibody against RAC1 (dilution: 1:300, Bioss, BJ, CHN).

Techniques: Expressing

Journal: Cells

Article Title: RAC1 , a Potential Diagnostic and Prognostic Marker for Diffuse Large B Cell Lymphoma

doi: 10.3390/cells11244039

Figure Lengend Snippet: Correlation between RAC1 expression and DLBCL clinical characteristics.

Article Snippet: Sections from paraffin-embedded tissues were then immunostained with a rabbit polyclonal antibody against RAC1 (dilution: 1:300, Bioss, BJ, CHN).

Techniques: Expressing

Journal: Cells

Article Title: RAC1 , a Potential Diagnostic and Prognostic Marker for Diffuse Large B Cell Lymphoma

doi: 10.3390/cells11244039

Figure Lengend Snippet: Logistic analyses of RAC1 expression and clinical characteristics of DLBCL.

Article Snippet: Sections from paraffin-embedded tissues were then immunostained with a rabbit polyclonal antibody against RAC1 (dilution: 1:300, Bioss, BJ, CHN).

Techniques: Expressing

Journal: Cells

Article Title: RAC1 , a Potential Diagnostic and Prognostic Marker for Diffuse Large B Cell Lymphoma

doi: 10.3390/cells11244039

Figure Lengend Snippet: RAC1 expression in patients with DLBCL according to different clinical stages.( A ) In the TCGA-DLBC dataset, RAC1 was upregulated in stage I/II as compared with that in stage III/IV. ( B ) RAC1 protein IHC staining in lymphadenitis and ( C ) DLBCL stage II, ( D ) stage III, ( E ) stage IV. Magnification 20 × 20, * p < 0.05. The staining of 3,3′-diaminobenzidine (DAB) was employed for RAC1 expression (yellow and brown) compared to negative sample (blue). Scale bar (bottom, right) = 100 μm.

Article Snippet: Sections from paraffin-embedded tissues were then immunostained with a rabbit polyclonal antibody against RAC1 (dilution: 1:300, Bioss, BJ, CHN).

Techniques: Expressing, Immunohistochemistry, Staining

Journal: Cells

Article Title: RAC1 , a Potential Diagnostic and Prognostic Marker for Diffuse Large B Cell Lymphoma

doi: 10.3390/cells11244039

Figure Lengend Snippet: Cox regression analyses of clinical features associated with DLBCL overall survival.

Article Snippet: Sections from paraffin-embedded tissues were then immunostained with a rabbit polyclonal antibody against RAC1 (dilution: 1:300, Bioss, BJ, CHN).

Techniques:

Journal: Current Issues in Molecular Biology

Article Title: Coptis chinensis Franch. Suppresses Invasive Pulmonary Aspergillosis by Augmenting NADPH-Dependent Neutrophil Extracellular Traps via Dual Modulation of Complement Activation and Gut Microbiota

doi: 10.3390/cimb48040424

Figure Lengend Snippet: Bayesian networks and KEGG suggest a CCF-biased reorganization toward CR3-SYK-NOX/NET pathways relative to IPA/AmB. The network analysis highlights recurrent CCF-associated hub genes, including Itgam, Syk, Rac2, and Cybb, together with pathway enrichment linked to oxidative and NET-related responses. ( A ) Hub gene networks identified in the IPA, CCF, and AmB groups by Bayesian network analysis. Genes were ranked by DEGREE value, with the top 10% defined as hub genes and marked in color; nodes with DEGREE >6 were placed at the core of the network. ( B ) KEGG pathway enrichment of hub genes in each group. Pathways were ranked by −log 10 ( p value). Bar colors represent different groups: IPA (dark blue), CCF (dark red), and AmB (light blue). The red box denotes the most significantly enriched KEGG pathway in the CCF group. ( C ) FPKM values of key CCF-regulated genes at +2, +4, and +6 dpi. Statistical analysis was conducted comparing all groups to the CCF group. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 indicate levels of statistical significance. ( D ) qPCR validation of key CCF-regulated genes in each group. Statistical analysis was conducted using one-way ANOVA, comparing all groups to the CCF group. * p < 0.05, ** p < 0.01, **** p < 0.0001 indicate levels of statistical significance. Data are presented as mean ± SD from n = 3 mice per group.

Article Snippet: Primary antibodies used in Western Blot included Beta Actin Monoclonal antibody (66009-1-Ig, Proteintech, Wuhan, China), NCF4/p40phox Rabbit pAb (A2096, Abclonal, Wuhan, China), Anti-NOX2/gp91phox Recombinant Rabbit Monoclonal Antibody (ET1611-44, Huabio, Hangzhou, China), RAC2 Rabbit pAb (A1139, Abclonal, China) and Anti-NCF1/p47phox Rabbit pAb (GB11724, Servicebio, Wuhan, China).

Techniques: Biomarker Discovery

Journal: iScience

Article Title: Preclinical efficacy of TZG in myofascial pain syndrome by impairing PI3K-RAC2 signaling-mediated neutrophil extracellular traps

doi: 10.1016/j.isci.2023.108074

Figure Lengend Snippet:

Article Snippet: Rabbit anti-RAC2 (dilution 1:1000, polyclonal) , Affinity Biosciences LTD. Melbourne, Australia , DF6273 RRID: AB_2838239.

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Gene Expression, Microarray, Software, Microscopy, Spectrophotometry, Sequencing, Fluorescence

Journal: Journal of Cell Death

Article Title: RTKN2 Induces NF-KappaB Dependent Resistance to Intrinsic Apoptosis in HEK Cells and Regulates BCL-2 Genes in Human CD4 + Lymphocytes

doi:

Figure Lengend Snippet: Western blot analysis of HEK RTKN2 stable cell lysates pre- and post-immunoprecipitation (IP) with RhoA and Rac2. A) Pre-IP following transfection with either Rac2 or RhoA, protein lysates were collected (48 h post-transfection) before the immunoprecipitation step (pre-IP) (Fig. 6A). The samples were denatured and run in reducing conditions on a 4%–20% gradient SDS PAGE gel, before transfer and immunoblotting with, anti-V5 (to detect RTKN2), anti-HA (Rac2) or anti-RhoA (RhoA). Bands were detected for all the proteins. B) Post-IP proteins lysates were then collected (48 h post-transfection) and immunoprecipitation performed overnight with anti-V5 (for RTKN2) and A/G sepharose. The samples (Post-IP) were denatured and run in reducing conditions on a 4%–20% gradient SDS PAGE gel, before transfer and immunoblotting with anti-V5 (to confirm IP), anti-HA or anti-RhoA (Fig. 6B). No band was detected at the correct size for either Rac2 or RhoA.

Article Snippet: The western blot was performed as described above using the antibodies, mouse-anti-V5 (RTKN2) (Invitrogen), rabbit-anti-RhoA (Cell Signaling) or rabbit-anti-HA (Rac2) (Zymed Laboratories).

Techniques: Western Blot, Stable Transfection, Immunoprecipitation, Transfection, SDS Page